Noregistration upload of files up to 250MB. Not available in some countries. Sporicidal efficacy of p. H adjusted bleach for control of bioburden on production facility surfacesp. H adjusted bleach was one of the agents used to disinfect contaminated public buildings in the USA following the 2. Bacillus anthracis spores. A USEPA fact sheet describes the preparation of p. H adjusted bleach by combining diluted sodium hypochlorite Na. Bleach Volume 55 Pdf Free' title='Bleach Volume 55 Pdf Free' />ORIGINAL ARTICLE Characterization of PhosphateFree Detergent Powders Incorporated with Palm C16 Methyl Ester Sulfonate C16MES and Linear Alkyl Benzene Sulfonic. OCl with a controlled amount of 5  acetic acid. This paper reports a modification of this procedure to qualify the use of p. H adjusted bleach for routine disinfection of cleanroom surfaces in pharmaceutical manufacturing facilities whenever a short contact time is desirable or there is a need for enhanced germicidal or sporicidal activity. Adjustment of p. H was obtained reproducibly with either acetic acid or HCl, confirming the feasibility of developing standard procedures for the controlled addition of acid to diluted Na. OCl solutions without compromising operator safety and convenience. Efficacy testing using spores from an in house isolate of Bacillus pumilus confirmed that Na. OCl solutions in the p. H 58 range have much greater sporicidal activity on surfaces than do unadjusted alkaline solutions p. H  1. 1. With a contact time of 0. Solutions of p. H adjusted Na. OCl are known to be less stable than unadjusted alkaline solutions. Stability studies were performed by monitoring sporicidal efficacy, level of free available chlorine FAC, and p. H. Testing included several Na. OCl concentrations and adjustment to different starting p. Hs. The efficacy of p. H adjusted solutions persisted in open containers for at least 1. FAC degradation occurred. In addition, solutions of 0. Na. OCl stored at room temperature protected from light retained efficacy for at least 4 weeks, indicating that short term storage of solutions is possible following p. H adjustment. The inorganic chemical degradation of p. H adjusted Na. OCl solutions generates chlorate ion, an undesirable by product. A comparison of chemical stability for 0. Na. OCl solutions adjusted to different initial p. Hs indicated that the least chlorate formation occurred with 0. Na. OCl. Keywords. How To Create A Virus Using Notepad Harmful Pdf on this page. Sporicide Cleanrooms Hypochlorous acid Bleach p. H adjustment Disinfection.